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Authors: Gokhale, Rajesh S.
Singh, Archana
Gotherwal, Vishvabandhu
Junni, Päivi
Vijayan, Vinaya
Tiwari, Manisha
Ganju, Parul
Kumar, Avinash
Sharma, Pankaj
Fatima, Tanveer
Gupta, Aayush
Holla, Ananthaprasad
Kar, Hemanta K.
Khanna, Sangeeta
Thukral, Lipi
Malik, Garima
Natarajan, Krishnamurthy
Gadgil, Chetan J.
Lahesmaa, Riitta
Natarajan, Vivek T.
Rani, Rajni
Title: Mapping architectural and transcriptional alterations in nonlesional and lesional epidermis in vitiligo
Publisher: Springer Nature
Publication Date: Aug-2017
Abstract: In vitiligo, chronic loss of melanocytes and consequent absence of melanin from the epidermis presents a challenge for long-term tissue maintenance. The stable vitiligo patches are known to attain an irreversible depigmented state. However, the molecular and cellular processes resulting in this remodeled tissue homeostasis is unclear. To investigate the complex interplay of inductive signals and cell intrinsic factors that support the new acquired state, we compared the matched lesional and non-lesional epidermis obtained from stable non-segmental vitiligo subjects. Hierarchical clustering of genome-wide expression of transcripts surprisingly segregated lesional and non-lesional samples in two distinct clades, despite the apparent heterogeneity in the lesions of different vitiligo subjects. Pathway enrichment showed the expected downregulation of melanogenic pathway and a significant downregulation of cornification and keratinocyte differentiation processes. These perturbations could indeed be recapitulated in the lesional epidermal tissue, including blunting of rete-ridges, thickening of stratum corneum and increase in the size of corneocytes. In addition, we identify marked increase in the putrescine levels due to the elevated expression of spermine/spermidine acetyl transferase. Our study provides insights into the intrinsic self-renewing ability of damaged lesional tissue to restore epidermal functionality in vitiligo.
Issue No: 1
Appears in Collections:NeuroImmunology II, Publications

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