Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1049
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dc.contributor.authorSehgal, Devinder-
dc.contributor.authorSori, Hema-
dc.contributor.authorJhelum, Hina-
dc.date.accessioned2020-07-21T06:04:14Z-
dc.date.available2020-07-21T06:04:14Z-
dc.date.issued2018-05-
dc.identifier.urihttp://hdl.handle.net/123456789/1049-
dc.description.abstractStreptococcus pneumoniae (pneumococcus) is a major bacterial pathogen that causes pneumonia and septicemia in humans. Pneumococci are cleared from the host primarily by antibody dependent opsonophagocytosis by phagocytes like neutrophils. Neutrophils release neutrophil extracellular traps (NETs) on contacting pneumococci. NETs immobilize pneumococci and restrict its dissemination in the host. One of the strategies utilized by pneumococci to evade the host immune response involves use of DNase(s) to degrade NETs. We screened the secretome of autolysin deficient S. pneumoniae to identify novel DNase(s). Zymogram analysis revealed 3 bands indicative of DNase activity. Mass spectrometric analysis led to the identification of TatD as a potential extracellular DNase. Recombinant TatD showed nucleotide sequence-independent endodeoxyribonuclease activity. TatD was associated with extracellular vesicles. Pneumococcal secretome degraded NETs from human neutrophils. Extracellular vesicle fraction from tatD deficient strain showed little NET degrading activity. Recombinant TatD efficiently degraded NETs. tatD deficient pneumococci showed lower bacterial load in lungs, blood and spleen in a murine sepsis model compared to wildtype strain, and showed less severe lung pathology and compromised virulence. This study provides insights into the role of a novel extracellular DNase in evasion of the innate immune system.en_US
dc.language.isoenen_US
dc.publisherNature.comen_US
dc.titleA novel extracellular vesicleassociated endodeoxyribonuclease helps Streptococcus pneumoniae evade neutrophil extracellular traps and is required for full virulenceen_US
dc.journalSci Rep .en_US
dc.volumeno8en_US
dc.issueno1en_US
dc.pages7985en_US
Appears in Collections:Molecular Immunology, Publications

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