Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1207
Title: A role for cell-autocrine interleukin-2 in regulatory T-cell homeostasis
Authors: Arimbasseri, Gopalakrishnan Aneeshkumar
Bal, Vineeta
George, Anna
Rath, Satyajit
Chawla, Amanpreet Singh
Khalsa, Jasneet Kaur
Dhar, Atika
Gupta, Suman
Umar, Danish
Keywords: FOXP3; IL2; Tregs; autocrine; paracrine
Issue Date: Jul-2020
Publisher: John Wiley & Sons Ltd
Abstract: Activated T-cells make both interleukin-2 (IL2) and its high-affinity receptor component CD25. Regulatory CD4 T-cells (Treg cells) do not make IL2, and the IL2-CD25 circuit is considered a paracrine circuit crucial in their generation and maintenance. Yet, all T-cells are capable of making IL2 at some stage during differentiation, making a cell-intrinsic autocrine circuit additionally possible. When we re-visited experiments with mixed bone marrow chimeras using a wide range of ratios of wild-type (WT) and IL2-/- genotype progenitors, we found that, as expected, thymic Treg cells were almost equivalent between WT and IL2-/- genotypes at ratios with WT prominence. However, at WT-limiting ratios, the IL2-/- genotype showed lower thymic Treg frequencies, indicating a role for cell-intrinsic autocrine IL2 in thymic Treg generation under IL2-limiting conditions. Further, peripheral IL2-/- naive CD4 T-cells showed poor conversion to inducible Tregs (pTregs) both in vivo and in vitro, again indicating a significant role for cell-intrinsic autocrine IL2 in their generation. Peripherally, the IL2-/- genotype was less prominent at all WT:IL2-/- ratios among both thymic Tregs (tTregs) and pTregs, adoptively transferred IL2-/- Tregs showed poorer survival than WT Tregs did, and RNA-seq analysis of WT and IL2-/- Tregs showed interesting differences in the T-cell receptor and transforming growth factor-beta-bone morphogenetic protein-JNK pathways between them, suggesting a non-titrating role for cell-intrinsic autocrine IL2 in Treg programming. These data indicate that cell-intrinsic autocrine IL2 plays significant roles in Treg generation and maintenance.
URI: http://hdl.handle.net/123456789/1207
Appears in Collections:Molecular Genetic, Publications

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