Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1282
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dc.contributor.authorGupta, Sarika-
dc.contributor.authorAhmad, Faiyaz-
dc.contributor.authorKumar, Ranjeet-
dc.contributor.authorRathaur, Sushma-
dc.date.accessioned2022-02-10T04:40:27Z-
dc.date.available2022-02-10T04:40:27Z-
dc.date.issued2020-11-
dc.identifier.urihttp://hdl.handle.net/123456789/1282-
dc.description.abstractAim: Identification of a 29 kDa heat stress protein in filarial parasite Setaria cervi and evaluation of its diagnostic potential against lymphatic filariasis. Methods and results: The Heat shock proteins (HSPs) were induced in filarial parasite S cervi by incubated at 42°C for 2 hours. The 10% SDS-PAGE of cytosolic extract showed several over-expressed bands. The MALDI-LC/MS analysis of 29 kDa band showed 100% similarity with Bm14-3-3 like protein 2. Multiple sequence alignment of Bm14-3-3 like protein 2 sequence with W bancrofti, Caenorhabditis elegans; Loa loa and Homo sapiens showed 100%, 86%, 83% and 78%, sequence similarity respectively. The antigenic efficacy of Sc14-3-3 protein was evaluated with different filarial sera using ELISA which showed cross-reactivity in order to Endemic Normal (EN) < Microfilaraemic (MF) < Chronic(CH) with IgG1 and EN < CH < MF in IgG4 ELISA. IgG1- and IgG4-specific immunoblotting with CH and MF sera further explicated its specific antigenic cross-reactivity. Conclusion: A 29 kDa heat shock protein of S cervi was identified as 14-3-3 protein having 100% homology to human filarial parasite B malayi. It showed strong reactivity with IgG1 and IgG4 subclass antibodies of W bancrofti-infected human sera suggesting that 14-3-3 protein could be used as a vaccine/ diagnostic marker.en_US
dc.language.isoenen_US
dc.publisherJohn Wiley & Sons Ltden_US
dc.subjectSetaria cervi; W bancofti; antigenicity; cross-reactivity; filariasis; heat shock protein.en_US
dc.titleIdentification of a HSP14-3-3 in Setaria cervi and its crossreactivity with W bancrofti-infected human seraen_US
dc.typeArticleen_US
dc.journalParasite Immunolen_US
dc.volumeno42en_US
dc.issueno11en_US
dc.pagese12777en_US
Appears in Collections:Molecular Sciences, Publications

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