Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1331
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dc.contributor.authorGeorge, Anna-
dc.contributor.authorRath, Satyajit-
dc.contributor.authorBal, Vineeta-
dc.contributor.authorD’Souza, Lucas-
dc.contributor.authorGupta, Sneh Lata-
dc.date.accessioned2022-04-06T06:12:17Z-
dc.date.available2022-04-06T06:12:17Z-
dc.date.issued2017-12-
dc.identifier.urihttp://hdl.handle.net/123456789/1331-
dc.description.abstractB-cell memory was long characterized as isotype-switched, somatically mutated and germinal centre (GC)-derived. However, it is now clear that the memory pool is a complex mixture that includes unswitched and unmutated cells. Further, expression of CD73, CD80 and CD273 has allowed the categorization of B-cell memory into multiple subsets, with combinatorial expression of the markers increasing with GC progression, isotype-switching and acquisition of somatic mutations. We have extended these findings to determine whether these markers can be used to identify IgM memory phenotypically as arising from T-dependent versus T-independent responses. We report that CD73 expression identifies a subset of antigen-experienced IgM+ cells that share attributes of functional B-cell memory. This subset is reduced in the spleens of T-cell-deficient and CD40-deficient mice and in mixed marrow chimeras made with mutant and wild-type marrow, the proportion of CD73+ IgM memory is restored in the T-cell-deficient donor compartment but not in the CD40-deficient donor compartment, indicating that CD40 ligation is involved in its generation. We also report that CD40 signalling supports optimal expression of CD73 on splenic T cells and age-associated B cells (ABCs), but not on other immune cells such as neutrophils, marginal zone B cells, peritoneal cavity B-1 B cells and regulatory T and B cells. Our data indicate that in addition to promoting GC-associated memory generation during B-cell differentiation, CD40-signalling can influence the composition of the unswitched memory B-cell pool. They also raise the possibility that a fraction of ABCs may represent T-cell-dependent IgM memory.en_US
dc.language.isoenen_US
dc.publisherJohn Wiley & Sons Ltden_US
dc.subjectCD40; CD73; IgM; T cells; age-associated B cells; memory.en_US
dc.titleCD73 expression identifies a subset of IgM + antigen-experienced cells with memory attributes that is T cell and CD40 signalling dependenten_US
dc.typeArticleen_US
dc.journalImmunologyen_US
dc.volumeno152en_US
dc.issueno4en_US
dc.pages602-612en_US
Appears in Collections:Mucosal Immunology, Publications

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