Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1351
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dc.contributor.authorSingh, Agam P-
dc.contributor.authorDhar, Suman K-
dc.contributor.authorPradhan, Sabyasachi-
dc.contributor.authorKalia, Inderjeet-
dc.contributor.authorRoy, Sourav Singha-
dc.contributor.authorSingh, Om P-
dc.contributor.authorAdak, Tridibes-
dc.date.accessioned2022-05-27T09:09:11Z-
dc.date.available2022-05-27T09:09:11Z-
dc.date.issued2019-09-
dc.identifier.urihttp://hdl.handle.net/123456789/1351-
dc.description.abstractProliferative cell nuclear antigen (PCNA) is the processivity factor for various DNA polymerases and it functions in response to DNA damage in eukaryotic system. Plasmodium falciparum contains two PCNAs, while PCNA1 has been attributed to DNA replication, the role of PCNA2 has been assigned to DNA damage response in erythrocytic developmental stages. Although a recent transposon mediated knockout strategy qualified pcna2 as a nonessential gene in Plasmodium berghei, a conventional homologous recombination-based knockout strategy has not been employed for this gene yet. Moreover, the cellular dynamics of PCNA2 in extraerythrocytic stages still remain elusive in Plasmodium. We attempted multiple times to knock out PbPCNA2 from the parasite genome using homologous recombination strategy without much success. However, we were able to generate PbPCNA2-GFP tagged transgenic parasites confirming that the pcna2 locus is amenable to genetic manipulation. The GFP-tagged parasites showed similar growth phenotype, compared to wild-type parasites, in both erythrocytic and sporogonic cycle, suggesting that tagging had no effect on parasite physiology. PbPCNA2 expression was also observed during the sporogonic cycle in midgut oocyst and salivary gland sporozoites. The PbPCNA2 expression was upregulated in the presence of DNA damaging agents like hydroxyurea and methyl methanesulphonate. Our inability to knock out PCNA2 suggested its essentiality in the parasite development and elevated expression during DNA damaging condition hint at a critical role of the protein in parasite physiology.en_US
dc.language.isoenen_US
dc.publisherInternational Union of Biochemistry and Molecular Biologyen_US
dc.subjectPlasmodium; DNA damage; DNA replication; PCNA; gene knockouten_US
dc.titleMolecular characterization and expression profile of an alternate proliferating cell nuclear antigen homolog PbPCNA2 in Plasmodium bergheien_US
dc.typeArticleen_US
dc.journalIUBMB Lifeen_US
dc.volumeno71en_US
dc.issueno19en_US
dc.pages1293-1301en_US
Appears in Collections:Infectious Disease, Publications

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