Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1373
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dc.contributor.authorPanda, Amulya Kumar-
dc.contributor.authorTariq, Mohammad-
dc.contributor.authorFatma, Sharmeen-
dc.contributor.authorTalegaonkar, Sushama-
dc.contributor.authorIqbal, Zeenat-
dc.contributor.authorNegi, Lalit Mohan-
dc.contributor.authorGoswami, Dinesh Giri-
dc.date.accessioned2022-06-13T09:45:39Z-
dc.date.available2022-06-13T09:45:39Z-
dc.date.issued2016-
dc.identifier.urihttp://hdl.handle.net/123456789/1373-
dc.description.abstractA receptor level interaction of etoposide with P-glycoprotein (P-gp) and subsequent intestinal efflux has an adverse effect on its oral absorption. The present work is aimed to enhance the bioavailability of etoposide by co-administering it with quercetin (a P-gp inhibitor) in dual-loaded polymeric nanoparticle formulation. Poly-lactic-co-glycolic acid (PLGA) nanoparticles were optimized for various parameters like o/w phase volume ratio, poly-vinyl alcohol concentration, PLGA concentration and sonication time. The cytotoxicity studies (MTT assay) revealed a 9- and 11-fold decrease in the IC 50 values for etoposide-loaded nanoparticles (ENP) and etoposide + quercetin dual-loaded nanoparticles (EQNP) when compared to that of free etoposide, respectively, and the results were further supported by florescent-activated cell sorter studies. The confocal imaging of the intestinal sections treated with ENP and EQNP containing fluorescent probe (rhodamine) showed the superiority of the EQNP to permeate deeper. Furthermore, pharmacokinetic studies on rats revealed that EQNP exhibited a 2.4-fold increase in bioavailability of etoposide than ENP with no quercetin. The developed loaded nanoparticles have the high potential to enhance the bioavailability of the etoposide and sensitize the resistant cells.en_US
dc.language.isoenen_US
dc.publisherInforma Healthcare USA, Inc.en_US
dc.subjectBioavailability; P-glycoprotein; PLGA; etoposide; nanoparticles; quercetinen_US
dc.titleNovel flavonoid-based biodegradable nanoparticles for effective oral delivery of etoposide by P-glycoprotein modulation: an in vitro, ex vivo and in vivo investigationsen_US
dc.typeArticleen_US
dc.journalDrug Deliven_US
dc.volumeno23en_US
dc.issueno2en_US
dc.pages500-511en_US
Appears in Collections:Product Development Cell Unit- II, Publications

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