Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/221
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dc.contributor.authorGokhale, Rajesh S-
dc.date.accessioned2014-11-20T09:10:44Z-
dc.date.available2014-11-20T09:10:44Z-
dc.date.issued2011-09-
dc.identifier.urihttp://hdl.handle.net/123456789/221-
dc.description.abstractThe life cycle of Dictyostelium discoideum is proposed to be regulated by expression of small metabolites. Genome sequencing studies have revealed a remarkable array of genes homologous to polyketide synthases (PKSs) that are known to synthesize secondary metabolites in bacteria and fungi. A crucial step in functional activation of PKSs involves their posttranslational modification catalyzed by phosphopantetheinyl transferases (PPTases). PPTases have been recently characterized from several bacteria; however, their relevance in complex life cycle of protozoa remains largely unexplored. Here we have identified and characterized two phosphopantetheinyl transferases from D. discoideum that exhibit distinct functional specificity. DiAcpS specifically modifies a stand-alone acyl carrier protein (ACP) that possesses a mitochondrial import signal. DiSfp in contrast is specific to Type I multifunctional PKS/fatty acid synthase proteins and cannot modify the stand-alone ACP. The mRNA of two PPTases can be detected during the vegetative as well as starvation–induced developmental pathway and the disruption of either of these genes results in non-viable amoebae. Our studies show that both PPTases play an important role in Dictyostelium biology and provide insight into the importance of PPTases in lower eukaryotes.en_US
dc.publisherPloS ONEen_US
dc.subjectBioinformaticsen_US
dc.titleTwo Functionally Distinctive Phosphopantetheinyl Transferases from Amoeba Dictyostelium discoideumen_US
dc.contributor.coauthorMohanty, Debasisa-
dc.contributor.coauthorNair, Divya R-
dc.contributor.coauthorGhosh, Ratna-
dc.contributor.coauthorManocha, Alzu-
dc.contributor.coauthorSaran, Shweta-
dc.keywordDistinctive Phosphopantetheinyl Transferases, Amoeba Dictyostelium discoideumen_US
dc.journalPLoS ONEen_US
dc.volumeno6en_US
dc.issueno9en_US
dc.pagese24262en_US
Appears in Collections:Bioinformatics Centre, Publications

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