Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/222
Full metadata record
DC FieldValueLanguage
dc.contributor.authorGokhale, Rajesh S-
dc.date.accessioned2014-11-20T09:58:40Z-
dc.date.available2014-11-20T09:58:40Z-
dc.date.issued2012-08-
dc.identifier.urihttp://hdl.handle.net/123456789/222-
dc.description.abstractGlycopeptidolipids (GPLs) are dominant cell surface molecules present in several non-tuberculous and opportunistic mycobacterial species. GPLs from Mycobacterium smegmatis are composed of a lipopeptide core unit consisting of a modified C(26)-C(34) fatty acyl chain that is linked to a tetrapeptide (Phe-Thr-Ala-alaninol). The hydroxyl groups of threonine and terminal alaninol are further modified by glycosylations. Although chemical structures have been reported for 16 GPLs from diverse mycobacteria, there is still ambiguity in identifying the exact position of the hydroxyl group on the fatty acyl chain. Moreover, the enzymes involved in the biosynthesis of the fatty acyl component are unknown. In this study we show that a bimodular polyketide synthase in conjunction with a fatty acyl-AMP ligase dictates the synthesis of fatty acyl chain of GPL. Based on genetic, biochemical, and structural investigations, we determine that the hydroxyl group is present at the C-5 position of the fatty acyl component. Our retrobiosynthetic approach has provided a means to understand the biosynthesis of GPLs and also resolve the long-standing debate on the accurate structure of mycobacterial GPLs.en_US
dc.publisherThe American Society for Biochemistry and Molecular Biology, Inc.en_US
dc.subjectBioinformaticsen_US
dc.titleRetrobiosynthetic Approach Delineates the Biosynthetic Pathway and the Structure of the Acyl Chain of Mycobacterial Glycopeptidolipidsen_US
dc.contributor.coauthorMohanty, Debasisa-
dc.contributor.coauthorSingh, Anil Kumar-
dc.contributor.coauthorMukherjee, Raju-
dc.contributor.coauthorChopra, Tarun-
dc.contributor.coauthorRavindran, Madhu Sudhan-
dc.contributor.coauthorChatterji, Dipankar-
dc.contributor.coauthorReyrat, Jean-Marc-
dc.contributor.coauthorVats, Archana-
dc.keywordGlycopeptidolipids, Mycobacterium smegmatisen_US
dc.journalJournal of Biological Chemistryen_US
dc.volumeno287en_US
dc.issueno36en_US
dc.pages30677-30687en_US
Appears in Collections:Bioinformatics Centre, Publications

Files in This Item:
File Description SizeFormat 
vats_jbc 2012 .pdf3.11 MBAdobe PDFView/Open    Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.