Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/234
Title: Solubilization of inclusion body proteins using n-propanol and its refolding into bioactive form
Authors: Garg, Lalit C
Singh, Surinder M
Sharma, Aparna
Upadhyay, Arun K
Singh, Anupam
Panda, Amulya K
Keywords: Protein Expression
Gene regulation
Issue Date: Jan-2012
Publisher: Elsevier Inc
Abstract: Inclusion bodies of recombinant human growth hormone (r-hGH) were isolated from Escherichia coli, enriched and solubilized in 100mM Tris buffer containing 6M n-propanol and 2M urea. Around 4 mg/ml of r-hGH from inclusion bodies were solubilized in 6M n-propanol-based buffer containing 2M urea. Existence of native-like secondary structure of r-hGH in 6M n-propanol solution was confirmed by CD and fluorescence spectra. Solubilized r-hGH was subsequently refolded by pulsatile dilution, purified to homogeneity and found to be functionally active. Tris buffer containing 6M n-propanol and 2M urea also effectively solubilized a number of proteins expressed as inclusion bodies in E. coli. Mild solubilization of inclusion body proteins, chaotropic effect of n-propanol at high concentration and kosmotropic effect at lower concentration helped in improved refolding of the solubilized protein. Around 40% of the r-hGH in the form of inclusion body aggregates was refolded into bioactive form while using n-propanol as solubilization agent. Solubilization with 6M n-propanol solution thus can be a viable alternative for achieving high throughput recovery of bioactive protein from inclusion bodies of E. coli.
URI: http://hdl.handle.net/123456789/234
Appears in Collections:Gene Regulation, Publications

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