Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/349
Title: Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans.
Authors: Tissenbaum, Heidi A
Mukhopadhyay, Arnab
Deplancke, Bart
Walhout, Albertha J M
Issue Date: Apr-2008
Publisher: Nature
Abstract: In order to determine how signaling pathways differentially regulate gene expression, it is necessary to identify the interactions between transcription factors (TFs) and their cognate cis-regulatory DNA elements. Here, we have outlined a chromatin immunoprecipitation (ChIP) protocol for use in whole Caenorhabditis elegans extracts. We discuss optimization of the procedure, including growth and harvesting of the worms, formaldehyde fixation, TF immunoprecipitation and analysis of bound sequences through real-time PCR. It takes approximately 10-12 d to obtain the worm culture for ChIP; the ChIP procedure is spaced out over a period of 2.5 d with two overnight incubations.
URI: http://hdl.handle.net/123456789/349
Appears in Collections:Molecular Aging, Publications

Files in This Item:
File Description SizeFormat 
nprot.2008.38.pdf297.02 kBAdobe PDFView/Open    Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.