Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/534
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dc.contributor.authorSuri, Anil-
dc.date.accessioned2015-05-26T11:15:37Z-
dc.date.available2015-05-26T11:15:37Z-
dc.date.issued2010-08-
dc.identifier.urihttp://hdl.handle.net/123456789/534-
dc.description.abstractBACKGROUND: Cervical cancer is a major cause of death among women worldwide, and the most cases are reported in the least developed countries. Recently, a study on DNA microarray gene expression analysis demonstrated the overexpression of heat shock protein 70-2 (HSP70-2) in cervical carcinoma cells (HeLa). The objective of the current study was to evaluate the association between HSP70-2 expression in cervical carcinogenesis and its potential role in various malignant properties that result in disease progression. METHODS: HSP70-2 expression was examined in various cervical cancer cell lines with different origins and in clinical cervical cancer specimens by reverse transcriptase-polymerase chain reaction (RT-PCR), flow cytometry, and immunohistochemistry (IHC) analyses. A plasmid-based, short-hairpin RNA approach was used specifically to knock down the expression of HSP70-2 in cervical tumor cells in vitro and in vivo to examine the role of HSP70-2 on various malignant properties. RESULTS: RT-PCR and IHC analyses revealed HSP70-2 expression in 86% of cervical cancer specimens. Furthermore, knockdown of HSP70-2 expression significantly reduced cellular growth, colony formation, migration, and invasion in vitro and reduced tumor growth in vivo. A significant association of HSP70-2 gene and protein expression was observed among the various tumor stages (P=.046) and different grades (P=.006), suggesting that HSP70-2 expression may be an indicator of disease progression. CONCLUSIONS: The current findings suggested that HSP70-2 may play an important role in disease progression in cervical carcinogenesis. Patients who had early stage disease and low-grade tumors had HSP70-2 expression, supporting its potential role in early detection and aggressive treatment modalities for cervical cancer management.en_US
dc.publisherAmerican Cancer Societyen_US
dc.titleGerm cell-specific heat shock protein 70-2 is expressed in cervical carcinoma and is involved in the growth, migration, and invasion of cervical cellsen_US
dc.contributor.coauthorGarg, Manoj-
dc.contributor.coauthorKanojia, Deepika-
dc.contributor.coauthorSaini, Shikha-
dc.contributor.coauthorSuri, Sushma-
dc.contributor.coauthorGupta, Anju-
dc.contributor.coauthorSurolia, Avadhesha-
dc.keywordheat shock protein 70-2, Cervical carcinoma, Germ cell-specific protein, Early detection, Invasionen_US
dc.journalCanceren_US
dc.volumeno116en_US
dc.issueno16en_US
dc.pages3785-3796en_US
Appears in Collections:Genes and Proteins, Publications

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