Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/796
Title: Role of proteasomal activity in the induction of acrosomal exocytosis in human spermatozoa
Authors: Gupta, Satish Kumar
Chakravarty, Sanchita
Bansal, Pankaj
Sutovsky, Peter
Issue Date: Mar-2008
Publisher: Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge
Abstract: Sperm-associated proteasomes have been suggested to play an important role during fertilization in animals. To delineate the role of these proteasomes during fertilization in humans, the present study reports proteasomal proteolytic activity both in noncapacitated and capacitated human spermatozoa, which is not altered in the presence of baculovirus-expressed recombinant human zona pellucida glycoprotein-3 (ZP3) and zona pellucida glycoprotein-4 (ZP4). However, inhibition of proteasomal proteolytic activity by clasto-lactacystin beta-lactone (CLBL) and Z-Leu-Leu-Leu-CHO (MG132), which are specific inhibitors of the 20S proteasomal core proteases, led to a significant (P < 0.05) inhibition of induction of acrosome reaction mediated by both recombinant human ZP3 and ZP4. Both inhibitors, however, failed to inhibit the induction of acrosomal exocytosis mediated by pharmacological agonist, calcium ionophore (A23187). The binding of recombinant human ZP3 and ZP4, labelled with fluorescein isothiocyanate, to the capacitated spermatozoa was not affected in the presence of proteasomal inhibitors. These observations suggest a role of the sperm proteasome in the induction of ZP3- and ZP4-mediated acrosomal exocytosis upstream of calcium signalling in humans.
URI: http://hdl.handle.net/123456789/796
Appears in Collections:Reproductive Cell Biology, Publications

Files in This Item:
File Description SizeFormat 
59.pdfResearch article (access limited)360.13 kBAdobe PDFView/Open    Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.