Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/880
Title: Modulation of naive CD8 T Cell response features by ligand density, affinity, and continued signaling via internalized TCRs
Authors: George, Anna
Balyan, Renu
Gund, Rupali
Ebenezer, Chitra
Khalsa, Jasneet Kaur
Verghese, Divya Anna
Krishnamurthy, Thyagarajan
Bal, Vineeta
Rath, Satyajit
Chaudhry, Ashutosh
Issue Date: Mar-2017
Publisher: The American Association of Immunologists, Inc
Abstract: T cell response magnitudes increase with increasing antigenic dosage. However, it is unclear whether ligand density only modulates the proportions of responding ligand-specific T cells or also alters responses at the single cell level. Using brief (3 h) exposure of TCR-transgenic mouse CD8 T cells in vitro to varying densities of cognate peptide-MHC ligand followed by ligand-free culture in IL-2, we found that ligand density determined the frequencies of responding cells but not the expression levels of the early activation marker molecule, CD69. Cells with low glucose uptake capacity and low protein synthesis rates were less ligand-sensitive, implicating metabolic competence in the response heterogeneity of CD8 T cell populations. Although most responding cells proliferated, ligand density was associated with time of entry into proliferation and with the extent of cell surface TCR downmodulation. TCR internalization was associated, regardless of the ligand density, with rapidity of c-myc induction, loss of the cell cycle inhibitor p27kip1, metabolic reprogramming, and cell cycle entry. A low affinity peptide ligand behaved, regardless of ligand density, like a low density, high affinity ligand in all these parameters. Inhibition of signaling after ligand exposure selectively delayed proliferation in cells with internalized TCRs. Finally, internalized TCRs continued to signal and genetic modification of TCR internalization and trafficking altered the duration of signaling in a T cell hybridoma. Together, our findings indicate that heterogeneity among responding CD8 T cell populations in their ability to respond to TCR-mediated stimulation and internalize TCRs mediates detection of ligand density or affinity, contributing to graded response magnitudes.
URI: http://hdl.handle.net/123456789/880
Appears in Collections:Mucosal Immunology, Publications

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