Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/982
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dc.contributor.authorGupta, Sarika-
dc.date.accessioned2017-09-12T10:17:51Z-
dc.date.available2017-09-12T10:17:51Z-
dc.date.issued2017-
dc.identifier.urihttp://hdl.handle.net/123456789/982-
dc.description.abstractBone morphogenetic protein-5 (BMP-5) is a member of the TGF receptor-b family with osteoinductive property. However, its physiological role in osteoblast differentiation is not defined. This study highlights the importance of BMP-5 in MC3T3E1 osteoblast differentiation. Pre-osteoblasts exposed to osteogenic media (ascorbic acid, 50 mg/ml and b-glycerophosphate, 10 mM) showed high protein expression of BMP-5 in cell lysates and cell culture supernatants, which peaked during early time-points of differentiation and declined with onset fmineralization. Attenuation of endogenous BMP-5 protein expression by RNA interference downregulated the expression of type I collagen (COLIA1), an early osteoblast differentiation marker but not osteocalcin, a late osteoblast differentiation marker. Further experiments to analyze the cell signaling components revealed that MP-5 modulates COLIA1 expression via p38-Runx2 axis involving Runx2 (Ser19) phosphorylation. These effects were also observed when recombinant BMP-5 was added to pre-osteoblast cultures reinforcing the fact that BMP-5 is a modulator of COLIA1 expression.We conclude that BMP-5 has stage-specific role to play during MC3T3E1 osteoblast differentiation in part by autocrine p38/ Runx2/COLIA1 signalingen_US
dc.publisherWileyen_US
dc.titleBone morphogenetic protein-5, a key molecule that mediates differentiation in MC3T3E1 osteoblast cell lineen_US
dc.contributor.coauthorVijayan, Viji-
dc.contributor.coauthorGupta, Sakshi-
dc.keywordBMP-5; Runx2; osteoblast; p38; differentiation; COLIA1en_US
dc.journalBiofactorsen_US
dc.volumeno43en_US
dc.issueno4en_US
dc.pages558-566en_US
Appears in Collections:Molecular Sciences, Publications

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